Browsing by Author "Özsar, Semin"
Authors:Güven, L; Özsar, Semin; Maraşlı, Nalan; Maraşlı, Şaban; Özcan, Ayla
Publisher and Date:(Scientific Technical Research Council Turkey-Tubitak, 2003)In this study a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be <0.01%. The detection limit of the assay was 2.5 ...