Browsing by Author "Özyiğit, İbrahim Ethem"
The modifier effects of chymotrypsin and trypsin enzymes on fluorescence lifetime distribution of "N-(1-pyrenyl)maleimide-bovine serum albumin" complex Authors:Özyiğit, İbrahim Ethem; Karakuş, Emine; Pekcan, Önder
Publisher and Date:(Pergamon-Elsevier Science LTD, 2016)Chymotrypsin and trypsin are the well known proteolytic enzymes, both of which are synthesized in the pancreas as their precursors the inactive forms; chymotrypsinogen and trypsinogen and then are released into the duodenum to cut proteins into smaller peptides. In this paper, the effects of activities of chymotrypsin and trypsin enzymes on fluorescence lifetime distributions of the substrat bovine serum albumin (BSA) modified with N-(1-pyrenyl)maleimide (PM) were examined. In the labeling study ...
Structural Analysis of Peptide Fragments Following The Hydrolysis of Bovine Serum Albumin by Trypsin and Chymotrypsin Authors:Özyiğit, İbrahim Ethem; Akten, Ebru Demet; Pekcan, Önder
Publisher and Date:(Taylor & Francis Inc, 2016)Peptide bond hydrolysis of bovine serum albumin (BSA) by chymotrypsin and trypsin was investigated by employing time-resolved fluorescence spectroscopy. As a fluorescent cross-linking reagent N-(1-pyrenyl) maleimide (PM) was attached to BSA through all free amine groups of arginine lysine and/or single free thiol (Cys34). Time-resolved fluorescence spectroscopy was used to monitor fluorescence decays analyzed by exponential series method to obtain the changes in lifetime distributions. After the ...