Browsing by Author "Kocazeybek, Bekir Sami"

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Citation Count: 0
C-reactive protein as a pre-procedural predictor of early and late outcomes of coronary angioplasty and intracoronary stent placement
(W.B. Saunders Co Ltd, 2001) Aytekin, Vedat; Aytekin, Saide; Çatakoğlu, Alp Burak; Kocazeybek, Bekir Sami; Demiroğlu, I. C. Cemşid; Demiroğlu, C
[Abstract Not Available]
Citation Count: 2
C-reactive protein as a pre-procedural predictor of early and late outcomes of percutaneous coronary interventions
(Springer New York, 2003) Aytekin, Saide; Çatakoğlu, Alp Burak; Aytekin, Vedat; Demiroğlu, Cemşid; Kocazeybek, Bekir Sami
To examine the predictive value of pre-procedural CRP level in patients undergoing percutaneous coronary intervention (PCI) regardless of having unstable or stable angina pectoris or myocardial infarction. Blood sampling for CRP measurement in patients undergoing PCI: 116 consecutive patients who underwent single vessel PCI were evaluated. Exclusion criteria were multilesion PCI, total occlusion, left ventricular ejection fraction < 30%, left bundle branch block and intercurrent inflammatory conditions known to be associated with an acute phase response. Major adverse coronary events (MACE) were defined as the occurrence of death, fatal or nonfatal myocardial infarction, and need for coronary artery revascularization with either bypass grafting or repeat angioplasty. End-points were assessed at hospital discharge, 30 days, 3 and 6 months following the index procedure. 62 (53%) patients had CRP levels < 0.5 mg/dl, and 54 (47%) had > 0.5 mg/dl. There were no significant difference in the occurrence of MACE in early in-hospital and 30 days follow up periods, between the two groups (0 vs. 5.5%) (p = ns) whereas the incidence of MACE after 3 months of the procedure was significantly different between the two groups (1.6 vs. 11%) (p < 0.05) and also after 6 months (9.5 vs 24.5%) (p < 0.05). The negative predictive value of CRP measurement is 98.4%. High levels of pre-procedural CRP show association with the higher incidence of MACE after 3 months of the follow-up period and negative CRP tests seems to have high predictive value to compare the patients who will be free of MACE after successful PCI. SciVal Topic Prominence
Citation Count: 6
Comparison of the BBL-mycobacteria growth indicator tube method with culture in the diagnosis of tuberculosis and evaluation of the resistance patterns of isolated strains to four major drugs
(Karger, 2002) Kocazeybek, Bekir Sami
The BBL-mycobacteria growth indicator tube system (MGIT) is used for a rapid detection of the presence of mycobacteria. Our study aimed to compare MGIT with the Lowenstein-Jensen (LJ) reference method in clinical samples with suspected pulmonary and extrapulmonary tuberculosis and to evaluate the primary and secondary resistance patterns by determining the resistances of the isolated strains to four major anti mycobacteria I drugs. 648 clinical samples from different clinics with suspected pulmonary or extrapulmonary tuberculosis based on clinical radiological histopathological and immunological findings were included in the investigation. The samples were first stained with Ziehl-Neelsen (ZN) and then cultured in LJ medium according to the standard bacteriological procedure and in the MGIT as recommended by the manufacturer. Conventional biochemical tests and p-nitro-alpha-acethylamino-beta-hydroxypropiophene of the Bactec system were used to identify the isolated mycobacterial strains. The susceptibilities to streptomycin isoniazid rifampicin ethambutol were tested by the BBL-MGIT antibiotic susceptibility test and the resistances of the strains found to be resistant to any of the drugs were confirmed by the agar proportion method. Mycobacterium spp. were isolated in 61 (9.4%) out of 648 samples. Eventually 58 out of 61 strains were classified as Mycobacterium tuberculosis and the other 3 as Mycobacterium tuberculosis complex. 32 of these were ZN positive. The growth time was determined as 12.2 days by the MGIT method and 24.1 days by the LJ method (p < 0.001). 29 strains were ZN negative. Their growth time was 23 days by the MGIT method and 37 days by the LJ method (p < 0.001). Drug resistance was detected in 23 (37.7%) of 61 cases (of whom 39 were new and 22 were former patients)
Citation Count: 2
The evaluation of microbial contamination in platelet concentrates prepared by two different methods
(Pergamon-Elsevier Science Ltd, 2001) Kocazeybek, Bekir Sami; Arabaci, Ümit; Akdur, Hülya; Sezgic, Metin; Erentürk, Selim
The microbial contamination of platelet concentrates (PCs) prepared by two different methods both with a high risk of bacterial contamination during preparation and storage were evaluated. For apheresis platelets the concentrates were obtained using the Haemonetics MCS 3P device. For the random method platelets were obtained by two phase centrifugation in the Heraeus Cryofuge 8500 I device using the Kansuk 3-way bags which permit storage for five days. 1620 platelet pheresis units prepared by apheresis and 9838 units prepared by the random method were included in the study. Of the 11458 PCs studied 32 (0.27%) were false positives and 24 (0.2%) were real positives. All of the positive results occurred in platelets prepared by the random method. C. xerosis and S. epidermidis S. hominis Alpha-hemolytic streptococci all flora of the skin were isolated in the contaminated concentrates. The risk of microbial contamination of PCs prepared both by apheresis and from whole blood continues at a low rate although the products were collected into specific bags following rules including appropriate disinfection of the skin correct centrifugation collection time and optimal storage conditions including temperature and agitation. These results again emphasize the importance of. obeying phlebotomy rules and hand disinfection of the person who collects the blood as well as the need for careful skin decontamination of the donor during donation. (C) 2001 Elsevier Science Ltd. All rights reserved.
Citation Count: 0
Investigation of transfusion transmitted viruses in cases clinically suspected of posttransfusion hepatitis with undetermined ethiology
(Pergamon-Elsevier Science Ltd, 2002) Kocazeybek, Bekir Sami; Arabaci, Ümit; Sezgiç, Metin
Transfusion transmitted viruses (TTV) were investigated in cardiac surgery cases who were previously transfused with blood and/or blood products and were suspected of having posttransfusion hepatitis (PTH) based on the results of physical examination clinical findings biochemical blood test results and in a smaller number on radiological results. They were identified as having non-A-C hepatitis based on serological or molecular test methods. In this study out of 90 cases suspected for PTH and non-A-C 78 (86.7%) were male 12 (13.3%) were female and their ages were between 17 and 67. Ninety healthy blood donors who donated blood for the first time and had never had a transfusion were selected as the control group. They had alanine aminotransferase (ALT) levels < 40 U were seronegative for hepatitis B virus (HBV) and hepatitis C virus (HCV). Seventy-seven were immune and 13 were seronegative for hepatitis A virus (HAV). In this study TTV-deoxyribonucleic acid (DNA) investigation was performed by the polymerase chain reaction (PCR) method suggested by Takahashi et al. with 5' GCT ACG TCA CTA ACC ACG TG 3'(T801) and 5' CTG CGG TGT GTA AAC TCA CC 3' (T935) primers. TTV-DNA was found to be positive in 21 (23.3%) of the patient group and 4 (4.4%) of the control group (p < 0.05). In the patients determined to be TTV-DNA positive the admission time following transfusion was a minimum of 3 and a maximum of 15 (average 7) weeks. The average ALT levels detected at the time of admission did not show a difference between TTV-DNA positive and negative cases (p > 0.05). However the ALT levels had a tendency to rise and reached their highest level nine weeks after transfusion in the TTV-DNA positive cases although in two cases the ALT levels decreased to normal value after the 13th week. During the 24 month follow up of the TTV-DNA positives all cases except one were positive at the end of this period. The results of this study are the same as those reported in the literature suggesting that TTV-DNA excluding the main viral agents which are known to cause PTH can be determined in transfused PTH or non-transfused asymptomatic patients in varying ratios. In order to define the epidemiological properties and hepatic-extrahepatic pathologies more clearly we have looked for evidence of the viral agent which probably contaminates both by transfusion and non-transfusion routes. It is suggested that in addition to the case groups in this study new clinical studies are necessary including transfused but non-PTH patients. (C) 2002 Elsevier Science Ltd. All rights reserved.
Citation Count: 15
Prospective evaluation of platelets prepared by single and random methods during 5 days of storage: aspects related to quality and quantity
(Pergamon-Elsevier Science Ltd, 2002) Kocazeybek, Bekir Sami; Arabaci, Ümit Önel; Akdur, Hülya; Sezgiç, Metin; Erentürk, Şaduman
Platelets which have an important role in hemostatic mechanisms and which were prepared by single and random methods were investigated to measure quantity and aggregation response during 5 days of storage. The aggregation response and quantitative values of platelet concentrates (PCs) 60 of which were prepared by a single donor method and 62 by a random method were investigated during the 1st 3rd and 5th days of storage. The single donor platelets (SDP) were obtained by using the MCS Plus apheresis device and the random donor platelets (RDP) were obtained by two-phase centrifugation in the Heraeus 8500i centrifuge device (during the first phase platelet rich plasma was obtained then platelet concentrate was obtained from this product) and were stored at 22 degreesC on a circular agitator. In addition pH PO2 PCO2 glucose and lactate values were measured in order to evaluate the effects of storage. The aggregation response was measured using adenosine diphosphate (ADP) epinephrine (EPN) collagen (COLL) and ristocetin (RIST). The cell count in mm(3) and the total cell count were also measured. The total cell counts and cells in mm(3) of the PCs which were prepared by the single donar method on the 1st 3rd and 5th days were: 3.11 x 10(11) 3.09 x 10(11) 3.07 x 10(11) and 292 x 10(3) 290 x 10(3) 289 x 10(3) and of those prepared by the random method were: 5.71 x 10(10) 5.69 x 10(10) 5.66 x 10(10) and 156 x 10(3) 153 x 10(3) 151 x 10(3). The mean aggregation responses of the PCs prepared by the two methods on the 1st 3rd and 5th days expressed as a 'X were: ADP: 94.8-93.2 81.6 78.7 44.3-8.2