Electrical activity regulates AChR gene expression via JNK PKC zeta and Sp1 in skeletal chick muscle

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Date

2001

Authors

Altiok, Nedret
Changeux, Jean-Pierre

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Elsevier Science Bv

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Abstract

Electrical activity of myotubes represses nicotinic acetylcholine receptor (AChR) gene expression. This effect is mimicked by okadaic acid and blocked by tetrodotoxin (TTX) or staurosporine in cultured myocytes [Altiok et al. EMBO J. 16 (1997) 717-725]. In this study we investigated the mechanism of this repression. We show that addition of exogenous phospholipase D (PLD) and C inhibits AChR expression in a manner which parallels that of okadaic acid. Furthermore okadaic acid caused an increase of the threonine phosphorylation of protein kinase C zeta (PKC zeta) and activator of transcription factor (ATF2) and a decrease of the phosphorylation of Sp1. All these effects were reversed by staurosporine and TTX also abolished ATF2 phosphorylation. These data reveal a possible involvement of PLD c-jun N-terminal kinase PKC zeta and Sp1 in the repression of AChR genes by electrical activity. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

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c-jun N-terminal kinase, Phospholipase D, Protein kinase C, Skeletal muscle, Sp1

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11

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Q1

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Q1

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Volume

487

Issue

3

Start Page

333

End Page

338