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Presence and localization of connexins 43 and 26 in cell cultures derived from myometrial tissues from nonpregnant and pregnant women and from leiomyomas

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Presence and localization of connexins 43 and 26 in cell cultures derived from myometrial tissues from nonpregnant and pregnant women and from leiomyomas.pdf (106.2Kb)
Date
2000
Author
Ciray, Haydar Nadir
Fu, Xin
Olovsson, Matts
Ahlsen, Goran
Shuman, Cheryl
Lindblom, Bo
Ulmsten, Ulf
Abstract
OBJECTIVE: Our objective was to study the appearance and distribution of connexins 43 and 26 in various human myometrial cell cultures. STUDY DESIGN: Scrape loading Western blotting and immunohistochemical techniques were applied to cultured cells derived from myometrial tissues obtained from nonpregnant and pregnant women (upper and tower uterine segments) and from leiomyomas (tumor and analogous myometrial tissues). RESULTS: Scrape loading revealed the presence or metabolic coupling in all tissues. indirect immunohistochemical studies showed membrane localization of connexin 43 in all myometrial cultures. Western blots and indirect immunohistochemical studies showed the presence and localization of the connexin 26 protein and associated gap junctions in tissues from myomas and from nonpregnant and pregnant women except for those derived from the upper segment of the pregnant uterus. CONCLUSION: These results show that human myometrial cultures express various gap junction proteins and that there are regional differences in expression of connexins in tissues from pregnant women.

Source

American Journal of Obstetrics and Gynecology

Issue

4

Volume

182

Pages

926-930

URI

https://hdl.handle.net/20.500.12469/257
https://doi.org/10.1016/S0002-9378(00)70348-6

Collections

  • Araştırma Çıktıları / WOS [1412]

Keywords

Gap junctions
Myometrium
Connexin

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DSpace software copyright © 2002-2015  DuraSpace
Contact Us | Send Feedback
Theme by 
@mire NV