Sorption and Desorption of Pva-Pyrene Chains in and Out of Agarose Gel

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Date

2012

Authors

Kara, Selim
Gacal, Burçin
Tunç, Deniz
Yağcı, Yusuf
Pekcan, Önder

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Springer/Plenum Publishers

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Abstract

In situ steady-state fluorescence (SSF) measurement technique was applied to investigation of pyrene labeled Poly(vinyl alcohol) (PVA-Py) molecules diffusion in and out of agarose gels. Gel samples with four different concentration of agarose were prepared. PVA-Py was synthesized by "click" chemistry method and dissolved in water to use in diffusion experiments. The results were analyzed by using Fickian type diffusion model, and it was found that sorption and desorption processes of PVA-Py molecules in and out of agarose gel have two distinct regions for short and long diffusion times. Sorption and desorption coefficients were measured and it was seen that the diffusion rates were much larger at short times and at lower agarose concentrations.

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Keywords

Agarose gel, Diffusion, Fluorescence, Poly(vinyl alcohol), Pyrene

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Citation

8

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Q3

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Volume

22

Issue

4

Start Page

1073

End Page

1080