A comparison of fluorescence and UV-visible spectrometry techniques for thermal phase transitions of agarose gels
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Date
2015
Authors
Arda, Ertan
Kara, Selim
Mergen, Ömer Bahadır
Pekcan, Önder
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Abstract
In this study thermoreversible phase transitions of high (HMP) and low (LMP) melting point agarose gels were investigated by using the UV-vis and fluorescence spectroscopy techniques. Pyranine was added to the aqueous solution of agarose as a fluorescence-probe. Transmitted light (I (tr)) and fluorescence emission (I (fl)) intensities from the gel samples with different agarose concentrations were monitored during the heating (gel-sol) and cooling (sol-gel) processes. For the both techniques gel-sol (T (gs)) and sol-gel (T (sg)) transition temperatures were determined from the first derivatives of the sigmoidal transition paths. It was observed that the critical transition temperatures obtained from UV-vis and fluorescence data slightly increased depending on the agarose type and concentration and those values were found to be in accord with each other. Transition activation energies were determined using the Arrhenius type equation and were found to be strongly correlated with the agarose content in the gel system. The produced gel-sol (Delta E (gs)) and sol-gel (Delta E (sg)) transition energies from the fluorescence data were found to be lower than that of the UV-vis data which was attributed to the temperature-dependent fluorescence quenching effect.
Description
Keywords
Agarose, Transition energy, Critical temperature, Fluorescence probe, Thermoreversible phase transition
Turkish CoHE Thesis Center URL
Citation
6
WoS Q
N/A
Scopus Q
Q2
Source
Volume
72
Issue
2
Start Page
157
End Page
175